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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Int J Med Microbiol. 2015 Mar 6;305(3):392–403. doi: 10.1016/j.ijmm.2015.03.002

FIG 5. Sequencing of the babA promoter region.

FIG 5

To further assess the loss of BabA expression, the babA promoter region was sequenced for several isolates using primer IR (Table 1). The −10 core promoter element (TATAAT), the +1 transcriptional start site (A), and the ATG translational start codon are boxed. The poly-A tract upstream of the −10 region and the CT repeat region downstream of the translational start site are underlined. In comparison to USU101 (13 A’s, 8 CT’s) the output strains differ in the number of repeats in either one or both of these regions. The lengths of poly-A tracts range from 13 to 15 and the CT repeat lengths range from 8 to 10. The addition of CT repeats, seen in all of the isolates, occurs within the coding region of babA and results in the pre-mature truncation of the protein.