TABLE 3.
Sequence Comparison of babA from Input and Output Strains
| Strain | Time post inoculation | PCR for babA | Western Blot for BabA | No. CT repeats* | No. As in PolyA tract# |
|---|---|---|---|---|---|
| USU101 | 0 mo. | + | + | 8 | 13 |
| H2 | 2 mo. | + | − | 10 | 14 |
| H2 | 16 mo. | + | − | 9 | 14 |
| H2 | 18 mo. | + | − | 9 | 15 |
| H2 | 24 mo. | + | − | 9 | 14 |
| H2 | 55 mo. | + | − | 9 | 13 |
| H1 | 52 mo. | + | − | 10 | 14 |
| H5 | 47mo. | + | − | 9 | 13 |
| H4 | 47mo. | − | − | babB | N/A |
| EH1 | 55 mo. | − | − | babB | N/A |
| EH3 | 55 mo. | + | − | 9 | 14 |
| EH4 | 52 mo. | + | − | 9 | 13 |
| EH5 | 55 mo. | − | − | babB | N/A |
*
babB indicates that no CT repeats were present because the gene was replaced by babB.
#
N/A indicates that this is not applicable because the poly- A tract was lost due to the replacement of babA by babB.