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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cytometry B Clin Cytom. 2014 Oct 23;88(1):6–20. doi: 10.1002/cyto.b.21196

Table 4.

Effect of Excluding Dead Cells from the Flow Cytometric Analysis, on the Amount of Background Signal obtained when using Negative Control Reagents

Negative Control 1
Total Sample
 Dead Cell Exclusion
% CASTs # CASTs / µl % CASTs # CASTs / µl
Mean 0.04% 0.11 0.01% 0.03
SD 0.06% 0.17 0.01% 0.08
n 63 63 63 63
Min 0.00% 0.00 0.00% 0.00
Max 0.38% 0.87 0.05% 0.58
Negative Control 2
Total Sample
 Dead Cell Exclusion
% CASTs # CASTs / µl % CASTs # CASTs / µl
Mean 0.02% 0.05 0.00% 0.00
SD 0.04% 0.09 0.00% 0.01
n 63 63 63 63
Min 0.00% 0.00 0.00% 0.00
Max 0.22% 0.38 0.02% 0.06