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. Author manuscript; available in PMC: 2016 Mar 19.
Published in final edited form as: Mol Cell. 2015 Mar 19;57(6):1099–1109. doi: 10.1016/j.molcel.2015.01.035

Figure 1. Transcription and translation of mntP are induced by MnCl2.

Figure 1

(A) Diagram of the yobD-mntP region. The numbering is as follows: +1 is the transcription start site, +225 corresponds to the AUG start codon, +270 shows the position of the fusion to the 15th amino acid.

(B) β-galactosidase activity for strains carrying chromosomal fusions with indicated yobD-mntP regions grown in LB medium and incubated without and with 400 μM MnCl2 for 1 h. Transcriptional fusions (ii), (iii) and (v) rely on the lacZ ribosome-binding site and translational fusions (i) and (iv) rely on the mntP ribosome-binding site.

(C-D) β-galactosidase activity for strains carrying the PmntP660-lacZ transcriptional (C) and the PLlacO-5′UTRmntP-lacZ translational (D) fusions grown in M9 glucose medium and incubated without or with indicated metals for 1 h.

For all β-galactosidase assays (B-D), the results are given in Miller units as the mean ± SDM of three independent samples.