Figure 2.

Silencing of SCN5A gene that encodes Na_v1.5 in LV cardiomyocytes of dog with chronic HF by virally delivered siRNA

A, RT-PCR analysis of mRNA abundance in control (fresh isolated cells), in cells infected with virus that contains cDNA for Na_v1.5 siRNA + GFP (Ad-GFP-Na_v1.5) and GFP alone (Ad-GFP). Upper panel: representative gel of such analysis performed at different times after the virus infection. Lower panel: summary data with statistical analysis of experiments similar to that shown in the upper panel obtained in 5–7 cells. Relative Na_v1.5 mRNA is given as relative to control density of bands. *P < 0.05, vs. control and GFP. B, Western blot analysis of Na_v1.5 protein abundance in control, and in Ad-GFP-Na_v1.5- and Ad-GFP-treated cells. *P < 0.001, vs. control and Ad-GFP. C, RT-PCR analysis of different sodium channel isoforms after transfection with Na_v1.5-siRNA (from 4 to 8 samples). *P < 0.05. Statistically significant changes in A, B and C, were evaluated by the ANOVA test followed by Bonferroni's post hoc test. Data are mean ± SEM.