Figure 7.

Silencing of SCN1A gene that encodes Na_v1.1 with adenovirus containing coding sequences for siRNA in cardiomyocytes from dogs with chronic HF

A, summary data of RT-PCR analysis of mRNA abundance (representative gels are shown in the inset at the upper right corner of the panel) in cultured dog cardiomyocytes. AdNC, adenovirus expressing non-specific siRNA as a control, Ad1.1siRNA, adenoviral construct expressing Na_v1.1 silencing sequences. B, summary data of Western blot analysis of Na_v1.1 protein abundance in cells treated with control virus (lanes 2–5 and 10 in the representative gels shown in the inset at the right upper corner of the panel) or Na_v1.1-siRNA (lanes 1 and 6–9, see inset). C, summary data of RT-PCR analysis of different Na⁺ channel isoforms after transfection with Na_v1.1-siRNA (from 4 to 8 samples). *Statistically significant changes in A, B and C were evaluated by the ANOVA test followed by Bonferroni's post hoc test. Data are mean ± SEM.