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. 2015 Feb 17;106(3):244–252. doi: 10.1111/cas.12600

Figure 2.

Figure 2

A925L and 925LPE3 cells harbor the EML4-ALK fusion gene and are sensitive to anaplastic lymphoma kinase (ALK)–tyrosine kinase inhibitors. (a) Multiplex RT-PCR for EML4-ALK was carried out. V, variant. (b) Break-apart FISH revealed that A925L and A925LPE3 cells have an ALK fusion gene. (c) Tumor cells were treated with crizotinib or alectinib for 72 h. The cell viability was determined by MTT assay. (d) Tumor cells were treated with crizotinib (1 μM) or alectinib (1 μM) for 1 h. The cell lysates were harvested and proteins and phosphorylation (P-) statuses were examined by Western blot analysis. STAT3, signal transducer and activator of transcription 3.