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. 2015 Feb 17;106(3):315–323. doi: 10.1111/cas.12601

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Folliculin (FLCN) immunostaining in normal kidney, and Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) H&E staining of a normal kidney. (b) Serial section of sample A immunostained for FLCN. Nuclei of distal tubules were strongly positive for FLCN. (c–h) Immunostaining for FLCN in BHD and sporadic tumors. Weak cytoplasmic staining was evident in tumors of BHD patients (c, e, h). Insets highlight distal tubules of non-tumor areas, showing immunoreactivity to FLCN. Sporadic renal tumors (d, f, h) showed strong nuclear staining.