Table 4.
Frequencies of exon mutations
| KRAS exon 2 (%) | KRAS exon 3 (%) | KRAS exon 4 (%) | NRAS exon 2 (%) | NRAS exon 3 (%) | NRAS exon 4 (%) | Total† | Method | |
|---|---|---|---|---|---|---|---|---|
| PRIME | 40 (440/1096) | 4 (24/638) | 6 (36/620) | 3 (22/637) | 4 (26/636) | 0 (0/629) | 17 | Sanger SURVEYOR |
| 20050181 | 45 (486/1083) | 4.4 (24/548) | 7.7 (41/534) | 2.2 (12/536) | 5.6 (30/540) | 0 (0/532) | 20 | Sanger SURVEYOR |
| 20020408 | 43 (184/427) | 4.8 (8/166) | 5.0 (9/180) | 4.2 (7/166) | 3.0 (5/168) | 1.1 (2/180) | 18 | Sanger‡ SURVEYOR |
| OPUS | 43 (136/315) | 6.8 | 9.3 | 6.8 | 5.1 | 0.8 | 26 | BEAMing |
| CRYSTAL | 37 (136/315) | 3.3 | 5.6 | 3.5 | 2.8 | 0.9 | 15 | BEAMing |
| PEAK | N/A | 4 (9/225) | 7 (17/223) | 5 (12/224) | 6 (13/225) | 0 (0/223) | 22 | Sanger SURVEYOR |
| FIRE-3 | N/A | 4.3 (21/431) | 4.9 (24/458) | 3.8 (18/464) | 2 (10/468) | 0 (0/458) | 16 | Pyrosequencing |
†
KRAS/NRAS mutation ratio in wild type KRAS exon 2.
‡
Next generation sequencers were used to confirm some of codon mutations.