Figure 6.

Development of a NEURON simulation based upon our hypothesis and parameters set by experimental data. (A) A computer model was developed using NEURON, which includes TRPV4 channels and allows an accumulation of Ca²⁺ into the cell. This is then linked to a K_Ca channel. Within the model, we can change osmolality and also block Ca²⁺ conductance. (B) Action potentials during hypertonic conditions (320 mOsm). (C) Decrease in action potential frequency when osmolality is reduced to 300 mOsm, and (D) further decreased action potential frequency at 270 mOsm. (E) Dose–response curve during changes in osmolality within the model.