Figure 1.

TA1 induces itch: the effect of pyrilamine pretreatment. Mice were treated s.c. with saline (Veh) or with TA1 (0.4, 1.32 and 4 μg·kg⁻¹) and observed for the time of onset and intensity of a scratching behaviour as described in Methods (A). Results are mean ± SEM. n = 10 in each group. **P < 0.01 versus Veh; ***P < 0.001 versus Veh. (B) Itch was also measured in mice pretreated with 10 mg·kg⁻¹ pyrilamine (i.p.) or saline (i.p.) 30 min before 0.4, 1.32 and 4 μg·kg⁻¹ TA1 or saline s.c. The intensity of itch after 10 min from TA1 (0.4, 1.32 μg·kg⁻¹) was reported. Results are mean ± SEM. n = 10 in each group. ***P < 0.001 versus Veh; ##P < 0.01 versus TA1 in the absence of pyrilamine.