Figure 2.

The NHT of CD1 mice is reduced by TA1: the effect of pyrilamine pretreatment. Mice were treated i.p. with saline (Veh) or TA1 (0.4, 1.32 and 4 μg·kg⁻¹) (A). After 15 min from Veh or TA1 injection their threshold to noxius heat (from 45.5 to 47.5°C) was measured as described in Methods. In another set of experiments, mice were pretreated with 10 mg·kg⁻¹ pyrilamine (i.p.) or Veh (i.p) 15 min before 1.32 μg·kg⁻¹ TA1 i.p. (B). The NHT of the mice was measured as described in ‘Methods’. Results are mean ± SEM of 10 mice per group. *P < 0.05 versus Veh; ***P < 0.001 versus Veh.