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. 2015 Feb 10;172(7):1869–1881. doi: 10.1111/bph.13031

Figure 1.

Figure 1

Effect of UFP-512 on Nrf2 expression in different fractions of HEK293t cells. Representative Western blots (upper) and relative quantitation (lower) of Nrf2 in the total protein of HEK293 cells (A); in the cytoplasmic fraction (B); in the nuclear fraction (C). After treatment with UFP-512 for 0, 2, 4, 8, 16 and 24 h in normoxia, the total, cytoplasmic and nuclear fractions of HEK293t cells were extracted for Western blots. Levels of Nrf2 were determined with anti-Nrf2 antibody. Lamin B was chosen as an internal standard for Western blot analysis of the nuclear protein. At least three independent experiments were carried out. *P < 0.05, **P < 0.01, ***P < 0.001, significantly different from 0 h. Note that there are no significant changes in the Nrf2 protein after UFP-512 treatment in whole cell protein, although UFP-512 tended to increase it. UFP-512 decreased Nrf2 density in the cytoplasmic fraction but increased it in the nuclear fraction, suggesting translocation of Nrf2 from cytoplasm to nucleus.