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. 2015 Feb 10;172(7):1869–1881. doi: 10.1111/bph.13031

Figure 7.

Figure 7

DOR-induced expression of Nrf2-regulated genes after H/R. The expression of Nrf2-targeted gene NQO1, HO-1, GCLC and GCLM (D) mRNAs in the HEK293t cells exposed to UFP-512 and/or naltrindole in H/R condition was assessed by quantitative real-time PCR. C, normoxic control. —, no treatment after H/R. U, UFP-512 during H/R. N, naltrindole during H/R. U + N, UFP-512 and naltrindole co-treatment during H/R. *P < 0.05, **P < 0.01, ***P < 0.005, significantly different from the normoxic control (C). #P < 0.05, ##P < 0.01, ###P < 0.005 significantly different from H/R alone. ◊P < 0.05, ◊◊P < 0.01, ◊◊◊P < 0.005, significantly different from UFP-512 and H/R stress. At least three independent experiments were carried out per group. Note that except for GCLC, all other genes increased their mRNA expression after DOR activation during H/R, an effect blocked by DOR antagonism with naltrindole.