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. 2015 Apr;145:1–10. doi: 10.1016/j.jphotobiol.2015.02.012

Fig. 4.

Fig. 4

Singlet oxygen production and oxidation of DNA. (A) Singlet oxygen and ICL formation: HeLa cells grown in the presence of 200 μM SIdU or SBrdU for 48 h were incubated for 1 h in PBS made in H2O or D2O and then irradiated with UVA (20 kJ/m2) followed by 15 Gy γ-rays. Analysis was by the alkaline comet assay following Proteinase K digestion. DNA breakage is expressed as comet tail moment. (B) Generation of 8-oxoguanine: HeLa cells were treated with 100 μM SBrdU or SIdU for 48 h and UVA irradiated. The presence of DNA 8-oxoguanine was revealed by single cell electrophoresis following digestion with recombinant OGG1 as indicated. Right panel: In a control experiment HeLa cells were treated with 10 mM KBrO3 for 1 h and analysed in the same way.