Table 1. Morphological characteristics of IVIg particles.
| Magnification | Size bin(nm) | AEDa) (Mean ± STDc)) |
Particle perimeter/AED (Mean ± STD) |
Aspect ratiob) (Mean ± STD) |
|||
|---|---|---|---|---|---|---|---|
| -T20d) | +T20e) | -T20 | +T20 | -T20 | +T20 | ||
| 52,000× | 1 - 20 | 19.3 ± 0.0f) | 16.7 ± 2.6 | 3.75 ± 0.0f) | 3.38 ± 0.74 | 0.47 ± 0.0f) | 0.68 ± 0.17 |
| 20 - 40 | 34.1 ± 3.9 | 28.0 ± 5.3 | 3.51 ± 0.61 | 3.52 ± 1.05 | 0.63 ± 0.16 | 0.64 ± 0.22 | |
| 40 - 60 | 49.3 ± 5.1 | 42.2 ± 5.4 | 3.61 ± 0.62 | 4.34 ± 0.89 | 0.62 ± 0.14 | 0.57 ± 0.14 | |
| 60 - 80 | 70.1 ± 5.8 | 68.5 ± 5.5 | 4.04 ± 0.79 | 4.45 ± 0.97 | 0.55 ± 0.14 | 0.60 ± 0.14 | |
| 80 - 100 | 89.1 ± 5.5 | 85.8 ± 6.2 | 4.13 ± 0.54 | 4.34 ± 0.84 | 0.59 ± 0.11 | 0.56 ± 0.13 | |
| 100 - 150 | 121.5 ± 14.1 | 117.7 ± 17.2 | 4.57 ± 1.02 | 5.01 ± 1.37 | 0.60 ± 0.17 | 0.57 ± 0.15 | |
| 150 - 200 | 171.5 ± 14.9 | 165.9 ± 4.5 | 5.16 ± 1.06 | 5.25 ± 0.89 | 0.58 ± 0.13 | 0.58 ± 0.10 | |
| 21,000× | 1 - 50 | 39.8 ± 6.4 | 49.9 ± 0.0f) | 3.36 ± 0.78 | 3.92 ± 0.0f) | 0.69 ± 0.18 | 0.60 ± 0.0f) |
| 50 - 100 | 74.0 ± 14.2 | 74.8 ± 15.4 | 3.72 ± 1.17 | 3.88 ± 1.04 | 0.58 ± 0.20 | 0.58 ± 0.17 | |
| 100 - 150 | 120.4 ± 16.1 | 120.2 ± 15.2 | 4.13 ± 1.04 | 4.77 ± 1.52 | 0.60 ± 0.18 | 0.50 ± 0.17 | |
| 150 - 200 | 167.3 ± 12.7 | 167.4 ± 15.8 | 4.82 ± 0.94 | 5.14 ± 1.71 | 0.53 ± 0.12 | 0.72 ± 0.18 | |
| 200 - 250 | 223.9 ± 15.2 | 209.2 ± 6.8 | 5.31 ± 0.88 | 5.54 ± 1.96 | 0.56 ± 0.13 | 0.48 ± 0.08 | |
| 250 - 450 | 325.2 ± 60.7 | 381.4 ± 33.3 | 5.71 ± 2.04 | 5.49 ± 0.81 | 0.55 ± 0.19 | 0.55 ± 0.13 | |
| 450 - 650 | 567.2 ± 59.1 | 581.4 ± 73.0 | 5.49 ± 1.62 | 6.35 ± 1.83 | 0.62 ± 0.18 | 0.64 ± 0.12 | |
| 6,500× | 1 - 400 | 305.0 ± 58.6 | 321.1 ± 53.9 | 3.88 ± 1.29 | 3.65 ± 0.96 | 0.59 ± 0.21 | 0.61 ± 0.19 |
| 400 - 800 | 530.4 ± 100.1 | 544.8 ± 101.8 | 4.62 ± 1.60 | 3.92 ± 1.31 | 0.57 ± 0.21 | 0.62 ± 0.22 | |
| 800 - 1200 | 963.2 ± 138.1 | 946.1 ± 109.4 | 5.55 ± 1.64 | 4.57 ± 1.17 | 0.54 ± 0.19 | 0.59 ± 0.17 | |
| 1200 - 1600 | 1383.2 ± 129.8 | 1417.2 ± 138.3 | 5.03 ± 1.54 | 5.34 ± 1.22 | 0.62 ± 0.16 | 0.60 ± 0.13 | |
| 1600 - 2000 | 1802.4 ± 106.8 | 1849.5 ± 121.0 | 5.95 ± 1.70 | 5.46 ± 1.32 | 0.50 ± 0.08 | 0.59 ± 0.15 | |
| 2000 - 2700 | 2399.9 ± 221.0 | 2295.3 ± 180.6 | 5.05 ± 2.77 | 7.15 ± 1.95 | 0.68 ± 0.27 | 0.65 ± 0.14 | |
| 2700 - 3400 | 3165.0 ± 111.3 | 2851.7 ± 113.4 | 3.88 ± 0.14 | 6.45 ± 1.11 | 0.64 ± 0.01 | 0.59 ± 0.08 | |
a)
AED, area equivalent diameter
b)
Ratio of minimum to maximum Feret diameter
c)
STD, standard deviation
d)
-T20, Tween 20 absent during agitation and freeze/thaw stress
e)
+T20, Tween 20 present during agitation and freeze/thaw stress
f)
Standard deviation is zero because only one particle fell into this size bin.