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. Author manuscript; available in PMC: 2015 Mar 27.
Published in final edited form as: Bone. 2014 Sep 28;71:63–75. doi: 10.1016/j.bone.2014.07.039

Fig. 4.

Fig. 4

DKK1-Ab enhanced chondrocyte- and osteoblast-specific marker gene expression in callus tissues of mice. Total RNA was extracted from callus tissues 7, 10, 14, 21 and28 days after fracture in the mice treated with or without DKK1-Ab. Expression of chondrocyte-specific genes was examined. Expression of Sox9 was increased at day 7 (A); expression of Col2a1 was increased at days 7, 10, and 14 (B); and expression of Col10a1 was increased at day 14, 21, and 28 (C). Expression of Runx2 and Osterix was increased at days 14, 21, and 28 (D and E) and expression of osteocalcin(OC) was increased at days 21 and 28 (F). Expression of Dkk1 and β-catenin was examined. Expression of DKK1 was decreased at days 7 and 10 (G) and expression of β-catenin was increased at days 7 and 10 (H). Data are presented as means ± SD. *p < 0.05, one-way ANOVA followed by Tukey’s test, n = 3.