DKK1-Ab had no significant effect on cartilage and bone volume of fracture callus in β-cateninPrx1ER mice. Histological analysis was performed on day 7, 10, 14 and 21 samples and showed that cartilage volume was increased at day 7 and woven bone was increased at days 14 and 21 in DKK1-Ab treated Cre-negative control mice (A–C). In contrast, DKK1-Ab had no significant effect on cartilage and woven bone volume in fracture callus of β-cateninPrx1ER mice (A–C). Black arrows: cartilage area and blue arrows: woven bone area. Data are presented as means ± SD. *p < 0.05, between DKK1-Ab and PBS groups (Cre-negative control mice);#p < 0.05, between Cre-negative and β-cateninPrx1ER groups (without DKK1-Ab); two-way ANOVA followed by Tukey’s test, n = 6.