Fig. 9.
DKK1-Ab had no significant effect on gene expression of fracture callus in β-cateninPrx1ER mice. Total RNA was extracted from callus tissues 7, 10, 14, 21 and 28 days after fracture. In Cre-negative control mice treated with DKK1-Ab, the expression of Sox9 and Col2a1 was increased at day 7 and 10 (A and B), Aggrecan (Agc1) expression was increased at day 14 (C); expression of Col10a1 was increased at days 14, 21, and 28 (D); and expression of Mmp9 and Mmp13 was increased at days 14 and 21 (E and F). InCre-negative control mice treated with Dkk1-Ab, the expression of Runx2 and Osterix was increased at days 14, 21, and 28 (G and H) and expression of osteocalcin (OC) was increased at days 21 and 28 (I). Expression of DKK1 was decreased at days 7 and 10 (J); and expression of β-catenin was increased at days 7 and 10 (K) in Cre-negative control mice treated with Dkk1-Ab. There was no significantly difference between DKK1-Ab and PBS treated groups in β-catenin cKO mice (A–K). Data are presented as means ± SD. *p < 0.05, between DKK1-Ab and PBS groups (Cre-negative control mice); #p < 0.05, between Cre-negative and β-cateninPrx1ER groups (without DKK1-Ab); two-way ANOVA followed by Tukey’s test, n = 3.