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Binding fluorescently labeled G4T4G4 PNA and ppG4T4ppG4 PNA to pGT4 plasmid: Lane 1, binding with G4T4G4 PNA; lane 2, binding with ppG4T4ppG4 PNA. Top panel: native supercoiled plasmid DNA (SC), gel was unstained, PNA fluorescence was detected. Middle panel: pGT4 digested with AlwN1 restriction enzyme following PNA binding, gel was unstained, only PNA fluorescence was detected. Bottom panel: same gel as in the middle panel stained with ethidium bromide (EtBr).
