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Binding G4T4G4 PNA to pGT4 and pCRBcl2. After binding plasmids were digested with HindIII and XhoI restriction enzymes and 32P-labeled. Autoradiography of 6% polyacrylamide gel is shown. Lane 1, pGT4 without PNA; lane 2, pGT4 with G4T4G4 PNA; lane 3, pCRBcl2 without PNA; lane 4, pCRBcl2 with G4T4G4 PNA; lane 5, pBR322/Msp1 32P-labeled molecular weight marker. PNA-shifted bands in lane 2 are marked with the bracket.
