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. 2015 Mar 27;10(3):e0121355. doi: 10.1371/journal.pone.0121355

Table 1. Estimated temperature profiles in 18 segmented parts of dunghill A.

Temperature [°C] b Longest consecutive period above a certain temperature [°C] c Reduction[%] d % of the volume of the dunghill (cumulative) f
Half ring <30 30–40 40–50 ≥50 T (days) Max Average
1 a 0.16
2 a 0.48 (0.64)
3 a 0.8 (1.44)
4 a 1.12 (2.56)
5 a 1.44 (4)
6 36 46 15 0 >40(15) 43 42 e 1.76 (5.76)
7 26 47 24 0 >40(17) 44 43 e 2.08 (7.84)
8 21 44 32 0 >40(32) 46 44 e 2.4 (10.24)
9 17 42 38 0 >40(38) 47 45 e 2.72 (12.96)
10 14 39 40 4 >40(44) 51 46 e 3.04 (16)
11 10 39 40 8 53(3) 53 53 100 3.36 (19.36)
12 6 38 33 20 ≥55(3) 56 55 100 3.68 (23.04)
13 5 36 31 25 ≥56(4) 58 57 100 4 (27.04)
14 3 33 33 28 ≥55(8) 59 58 100 4.32 (31.36)
15 1 35 30 31 ≥57(7) 61 59 100 4.64 (36)
16 1 34 30 32 ≥55(10) 62 60 100 4.96 (40.96)
17 1 31 31 34 ≥55(11) 64 60 100 5.28 (46.24)
18 1 26 35 35 ≥56(11) 65 62 100 5.6 (51.84)
19 1 24 34 38 ≥58(11) 67 63 100 5.92 (57.76)
20 1 23 33 40 ≥56(12) 68 64 100 6.24 (64)
21 1 23 32 41 ≥57(12) 69 65 100 6.56 (70.56)
22 1 22 31 43 ≥58(12) 70 66 100 6.88 (77.44)
23 1 20 31 45 ≥59(12) 71 67 100 7.2 (84.64)
24 a 7.52 (92.16)
25 a 7.84 (100)

aTemperature profiles in the segmented parts 1, 2, 3, 4, 5, 24 and 25 fell outside the scope of the two measurement locations in the dunghill (see Fig. 5). These are therefore outside the range of validity of the temperature profile model.

bFor each segmented part, the number of days that the estimated temperature in the dunghill fell within a certain temperature interval during the 97 days of the experiment is presented.

cThe combination of the minimum daily temperature (T) with the longest consecutive time interval (days) that could achieve the maximum reduction percentage. In all cases, the highest temperature fell within this period. For the longest consecutive time period also the maximum and the average temperature are determined.

dEstimated reduction percentage of C. burnetii in the dunghill according to comparison with described decimal reduction time (DRT) in milk, as described by Enright et al. [21] and extrapolated using Equation 4.

eFor the segmented parts 6–10, the reduction percentage of C. burnetii could not be quantified based on the calculated temperature profiles. Reduction percentages in these segmented parts are less than 100% when compared to DRT of C. burnetii in milk [21]. Nevertheless, based on DRT in goat manure (see Table 3), survival of C. burnetii is just above 3 hours at a temperature of 40 degree Celsius. Therefore, total reduction of C. burnetii in the segmented parts 6–10 might also be possible.

fFor each segmented part, its contribution (%) to the total volume of the dunghill is presented. Also, the cumulative percentage is presented.