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. 2015 Mar 27;10(3):e0122258. doi: 10.1371/journal.pone.0122258

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 2 — Mouse bone marrow (BM) cells were collected from femurs and humeri 24 h after irradiation. (A) Flow cytometric analysis shows frequencies of live lineage-negative and lineage-dim (Lin-/dim) stem and progenitor cells in vehicle- or DT3-treated and sham or 7 Gy TBI mouse BM. Samples were pooled from 6 mice in the same group. Results were from one representative experiment of two independent experiments. (B) Clonogenicity of mouse BM cells was quantified in standard semisolid cultures in triplicate. Colonies were counted 10 days later. Results were from one representative experiment of two independent experiments (N = 6 mice /point/experiment). Means ± SD. **, p < 0.01, DT3-treated vs. vehicle-treated controls. (C) Clonogenicity of human CD34+ cells was quantified in standard semisolid cultures after 2 Gy or 4 Gy irradiation in triplicate. Colonies were counted 14 days later. Results were from one representative experiment of three independent experiments. Means ± SD. *, p<0.05, **, p < 0.01, DT3-treated vs. vehicle-treated controls.