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. 2015 Mar 27;10(3):e0122258. doi: 10.1371/journal.pone.0122258

Fig 6. An anti-IL-1β antibody protected CD34+ cells from radiation-induced apoptosis.

Fig 6

The anti-IL-1β antibody (0.2 μg/mL) or the same amount of nonspecific IgG as a control (CT) were added into CD34+ cell culture 1 h before sham- or 2 Gy radiation. (A) Cells were analyzed by flow cytometry with apoptotic and cell death marker Annexin-V and 7AAD staining at 24, 48, and 72 h after irradiation. Frequency of apoptosis (Annexin-V and 7AAD positive) cells is shown. (B) Clonogenic assay on survival of human hematopoietic progenitor cells was performed 1 day after irradiation. Colonies were counted at 14 days of culture. Colony generation was inhibited by radiation, and this inhibition was ameliorated by neutralization of IL-1β bioactivity using anti-IL-1β antibody. * p<0.05, **p<0.01; mean ± SD. Anti-IL-1β antibody vs. IgG control.