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. 2015 Jan 17;18(5):pyu028. doi: 10.1093/ijnp/pyu028

Figure 4.

Figure 4.

Pharmacological inhibition or genetic deletion of CB1Rs provides protection against ethanol-induced inhibition of Arc expression in the neonatal mouse brain. (a) Hippocampal and neocortical total extracts from the four treatment groups (S+V, E+V, S+SR, and E+ SR) were processed for Western blot to analyze the levels of Arc protein (n = 10 pups/group; ***p < 0.001 vs. S+V; #p < 0.001 vs. E+V). (b) To determine the Arc protein levels in saline and ethanol-treated CB1RWT and KO P7 mice samples, the hippocampal and neocortical total extracts were subjected to Western blot analyses. The representative blots are shown for the hippocampal and cortical cytosolic extracts (n = 10 pups/group; ***p < 0.001 vs. S+CB1RWT; #p < 0.001 vs. E+CB1RWT; @p < 0.001 vs. S+CB1RWT). Two-way ANOVA with Bonferroni’s post hoc tests was used for statistical analysis. Each point is the mean ± SEM. HP, hippocampus; NC, neocortex.