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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Stem Cells. 2015 Apr;33(4):1200–1212. doi: 10.1002/stem.1934

Figure 4. BMSC Exposure Enhances Purine Metabolism In Human Th1 Cells In Vitro.

Figure 4

Human Th1 cells from 3 different donors were co-cultured with clinical grade BMSC from 3 different donors in the presence of ATP (100μM) for 2 hrs at a 1:1 ratio. Some culture conditions included the inhibitor for CD73 (Adenosine 5’-(α,β-methylene) diphosphate sodium; 100μM). The supernatants were harvested and adenosine measured by GC-MS/MS._Representative GC-MS/MS graphs (A) and cumulative adenosine production in the supernatant from three experiments (B). The co-culture was repeated in a phosphate free reaction buffer and the amount of phosphate released in the supernatant was measured using Malachite Green Phosphate detection assay (C). P values were obtained by performing a student t- test where *=P<0.05, **=P<0.01 and ***=P<0.001.