Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Hepatology. 2015 Mar 9;61(4):1216–1226. doi: 10.1002/hep.27592

Fig. 2. Effect of high fat diet and cholic acid on SphK2 expression and enzymatic activity in liver.

Fig. 2

Wild type (WT) and SphK2−/− (KO) mice (male, 20-week old) were fed normal chow diet (ND; solid bar), high fat diet (HFD; checkered bar), or HFD containing 1% cholic acid (HFD+CA; shaded bar) for two weeks. (A) The mRNA levels of SphK2 in the liver of WT mice were determined by real time RT-PCR and normalized using GAPDH as internal control. (B) The protein expression levels of SphK2 in the liver of WT and SphK2−/− mice were determined by Western Blot analysis and normalized using β-tubulin as loading control. (C) The enzyme activities of SphK2 were measured using [γ32P]-ATP. *P<0.05, **P<0.01, statistical significance relative to control group.