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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Hepatology. 2015 Mar 9;61(4):1216–1226. doi: 10.1002/hep.27592

Fig. 5. Effect of S1PR2 on hepatic histone acetylation levels and mRNA levels of key genes involved in hepatic lipid metabolism.

Fig. 5

(A) Nuclear extracts of primary hepatocytes isolated from Wild type (WT) and S1PR2−/− mice were prepared as described in “Methods”. The protein levels of SphK2, H3K9ac, H4K5ac, H2BK12ac and total H3 were determined by Western blot analysis. Representative images are shown. (B) Total RNA was isolated from the livers of WT (solid bar) or S1PR2−/− (checkered bar) mice (male, 20-week old). The mRNA levels of key genes involved in lipid metabolism were determined using real time RT-PCR and normalized using β-actin. Abbs: SREBP-1c: sterol regulatory element-binding protein-1c; FAS: fatty acid synthase; LDLR: low-density lipoprotein receptor; CYP27A1: sterol 27-hydroxylase; CYP7A1: cholesterol 7 α-hydroxylase; BSEP: bile salt export pump; FXR: farnesoid X receptor. BECN1: autophagy-related gene (Atg) 6; PPARγ: peroxisome proliferator-activated receptor γ. *P<0.05, **P<0.01, ***P<0.01, statistical significance relative to WT mice, n=5–8. (C and D) The S1P and DHS1P levels in the cytosol and nucleus of mouse primary hepatocytes isolated from WT and S1PR2−/− mice were measured using mass spectrometry as described in “Methods”. *P<0.05, statistical significance relative to WT.