Table 1.

Thermodynamic parameters for the binding of WW1 domain of WWOX to PPXY-containing peptides derived from various putative ligands

Ligand	Sequence	Kd / μM	ΔH / kcal.mol−1	TΔS / kcal.mol−1	ΔG / kcal.mol−1
ErbB4		179 ± 10	−7.85 ± 0.80	−2.73 ± 0.83	−5.12 ± 0.03
p73		107 ± 9	−16.65 ± 0.78	−11.23 ± 0.82	−5.42 ± 0.05
PTCH1		278 ± 19	−10.17 ± 0.62	−5.31 ± 0.57	−4.86 ± 0.04
PTPN14		763 ± 62	−5.96 ± 0.72	−1.70 ± 0.77	−4.26 ± 0.04
SMAD7		272 ± 29	−22.60 ± 0.99	−17.73 ± 1.05	−4.87 ± 0.06
TMG2		202 ± 23	−14.15 ± 0.49	−9.10 ± 0.43	−5.05 ± 0.07
WBP1		368 ± 44	−17.55 ± 0.78	−12.86 ± 0.85	−4.69 ± 0.07
WBP2		133 ± 18	−15.15 ± 0.49	−9.85 ± 0.42	−5.30 ± 0.08

The consensus residues within the PPXY motif of each peptide are colored red for clarity. All parameters were obtained from ITC measurements at 25°C and pH 7. Binding stoichiometries were fixed to unity to improve the accuracy of thermodynamic parameters. Errors were calculated from at least three independent measurements to one standard deviation.