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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Stem Cells. 2015 Apr;33(4):1267–1276. doi: 10.1002/stem.1914

Figure 4. Delayed meiosis initiation and progression in Taf4b −/− testes.

Figure 4

(A) γH2AX immunofluorescence (green) of P19, P23 and P30 testis sections from wild type and Taf4b −/− mice. P30 Taf4b −/− seminiferous tubule phenotypic categories: (1) normal, (2) partial absence of spermatocytes, (3) having spermatids undergoing spermiogenesis but lacking spermatocytes, (4) completely devoid of meiotic spermatocytes and spermatids. DAPI (blue) * Denotes leptotene/zygotene stage spermatocytes with diffuse γH2AX staining, ** denotes pachytene stage spermatocytes with XY-body γH2AX foci. Scale bars = 100 μm. Quantitative analysis of γH2AX staining and meiotic progression during the first wave of spermatogenesis in wild type (B) and Taf4b −/− (C) testes. Tubules containing multiple meiotic cell types are categorized by furthest stage. Between 100 and 200 tubules were counted for each data point.