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Flow cytometric quantification of retained, viable ASCs. (A) Representative FACS plot demonstrating gating for sorting of GFP+ ASCs at day 5 post-grafting after retrieval from explanted fat grafts. (B) Progressive decline in percentage of ASC retrieval from digested grafts, measured by quantitative flow cytometry over time. Of the original number of ASCs injected with fat, 44% were retrieved on day 1, 6% on day 5, and 2% on day 10 post-grafting.
