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qRT-PCR analysis of ASC gene expression over time demonstrated an initial downregulation from day 0 (in vitro) to day 1 post-grafting for several genes, with the notable exception of VEGFA. A trend toward increasing expression was observed, however, with significantly higher expression seen at day 10 compared to day 5 for all genes studied (*p < 0.05, **p < 0.01).
