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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Bone Miner Res. 2015 Apr;30(4):723–732. doi: 10.1002/jbmr.2378

Figure 3. Osteonectin levels and gain in trabecular bone is higher in haplotype B knock-in mice.

Figure 3

(A) Osteonectin mRNA was quantified by qRT-PCR using RNA isolated from the femurs of 6-8 week old ONA/A and ONB/B mice. Osteonectin mRNA levels were normalized to 18s RNA (N=4 mice/ haplotype, * p<0.05 different from haplotype A). (B) Western blotting for osteonectin and β-actin in lysates from long bones of ONA/A and ONB/B mice (*p<0.05 different from haplotype A, N=3 mice/group). (C, D) μCT analysis of changes in femoral bone from 10 to 20 weeks of age in ONA/A and ONB/B mice. Trabecular bone volume (BVF), trabecular bone number (Tb.N) trabecular bone spacing (Tb.Sp.). Total (periosteal) area (Tt.Ar), marrow area (Ma.Ar), cortical area (Ct.Ar). *p<0.05 different from haplotype A, N=4-9 mice/group.