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. Author manuscript; available in PMC: 2016 Mar 24.
Published in final edited form as: Cell Rep. 2015 Mar 12;10(11):1828–1835. doi: 10.1016/j.celrep.2015.02.040

Figure 2. Monoclonal Enrichment of Erbb3-Deficient Stem Spermatogonia.

Figure 2

(A) CRISPR/Cas9-targeted Erbb3-mutations (exon 2) in clonally expanded rat spermatogonial lines.

(B) Relative abundance of ERBB3 (upper arrowhead) and TUBA1a (lower arrowhead) in wildtype (colony D4; WT) and Erbb3-deficient (colony B9; KO) spermatogonial lines.

(C) Left: Cultures of clonally expanded Wildtype (colony D4) and Erbb3 Knockout (colony B9) spermatogonia expressing tgGCS-EGFP. Scale, 50 μm. Right: Growth rates of clonally expanded Wildtype (WT) and Erbb3 Knockout (KO) spermatogonial lines. See also Figure S3.

(D) NRG1-dependent development of differentiating spermatogenic cells from Wildtype and Erbb3 Knockout spermatogonial lines.

(E) Spermatogenic cells from colonies D4 (Wildtype) and B9 (Erbb3 Knockout) analyzed in panel D. tgGCS-EGFP (green), ZBTB16 antibody (Red). Scale, 40 μm.