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. 2015 Mar 27;10(3):e0121512. doi: 10.1371/journal.pone.0121512

Fig 1. Design of the mouse experiments.

Fig 1

In Experiment I, four ΔdbpAB/dbpAB (group 2), eight ΔdbpAB/dbpA (group 3), eight ΔdbpAB/dbpB (group 4), two ΔdbpAB (group 5) infected animals and two uninfected control animals (group 1) were killed at seven weeks of infection. In Experiment II, 16 infected animals (groups 4 and 5) were treated with ceftriaxone and 16 (groups 6 and 7) with ceftriaxone and anti-TNF-alpha. The ceftriaxone treatment was started at two weeks (25 mg/kg twice a day for 5 days) and the anti-TNF-alpha treatment at seven weeks of infection (10 mg/kg once a week for 4 weeks). Ear biopsy samples were collected at 6 and 9 weeks of infection to monitor the dissemination of the infection. In Experiment III, mice were killed at two weeks to study infection kinetics and bacterial load in joints. In Experiment IV, eight infected animals were treated with ceftriaxone at six weeks of infection (groups 14 and 15).