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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Nat Neurosci. 2015 Mar 2;18(4):501–510. doi: 10.1038/nn.3960

Figure 3. DRP1 phosphorylation regulates mitochondrial morphology and stem cell marker expression.

Figure 3

a. Immunofluorescent staining of mitochondria by TOM20 in 387 and 3565 non-brain tumor initiating cells (non-BTICs) transduced by lentiviral control vector or a DRP1S616E/S637A double mutant. b. Mitochondria morphology was assessed from 120 cells of three different experiments. Data are displayed as mean ± s.e.m. (387, fragmented: p = 0.0009, tubular: p = 0.0002; 3565, fragmented: p = 0.0030, tubular: p = 0.0016; statistical significance determined by Student’s t-test; n = 3). c. Immunoblot analysis of DRP1 protein in 387 and 3565 non-BTICs expressing control vector or a DRP1S616E/S637A double mutant. Images were cropped for presentation. Full-length blots are presented in Supplementary Fig. 10. d, e. 387 non-BTICs were transduced with vector encoding a DRP1S616E/S637A double mutant or control vector. Three days after infection, total RNA was isolated and cDNA was synthesized by reverse transcription. The mRNA levels of indicated genes were detected by real-time qPCR. Data are displayed as mean ± s.e.m. (387: Olig2, p = 0.0072; Oct4, p = 0.0097; Nanog, p = 0.0087; Nestin, p= 0.0309; Pou3f2, p= 0.0067; CD133, p = 0.0219; SSEA1, p = 0.0041; GFAP, p = 0.0095; MAP2, p = 0.0001. 3565: Olig2, p = 0.0334; Oct4, p = 0.0097; Nanog, p = 0.0074; Pou3f2, p = 0.0022; CD133, p = 0.0093; SSEA1, p = 0.0251; GFAP, p = 0.0164; MAP2, p = 0.0020. Statistical significance determined by Student’s t-test; n = 3).