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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Mol Reprod Dev. 2015 Jan 14;82(3):167–181. doi: 10.1002/mrd.22454

Figure 7.

Figure 7

Human Na,K-ATPase α4 expression does not affect spontaneous acrosome reactions in transgenic mice. Epididymal sperm from wild type (WT) and transgenic mice (T hα4) were incubated in modified Tyrode's medium that does not support capacitation (non-capacitated, NC), or in modified Tyrode's medium supplemented with calcium, bicarbonate, and bovine serum albumin to support capacitation (C). After incubation at 37°C for the indicated times, the acrosome status of the cells was measured using Coomassie blue G-250 stain. A minimum of 350 cells was scored for each time point per experiment. Acrosome-reacted spermatozoa with no label were expressed as a percentage of the total amount of cells counted. Bars are the means ± standard errors of three experiments.