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. 2015 Mar 27;11(3):e1004750. doi: 10.1371/journal.ppat.1004750

Fig 9. Reduced neuroinvasiveness of NS5-M19A—mutated JEV in challenged mice.

Fig 9

(A) Survival in C57BL/6 mice infected with 103 or 105 PFU JEV-WT or JEV-NS5-M19A by an intraperitoneal (i.p.) plus i.c. route. The animal number (n) and survival rate for each group are shown. (B) Plaque-forming assay of virus titers in brain tissues of mice inoculated with JEV-WT or JEV-NS5-M19A (105 PFU) (n = 3). (C-E) RT-qPCR of relative JEV RNA (C), IL-6 (D), and TNF-α (E) mRNA levels in brain tissues (n = 3). (F) ELISA of IL-6 protein levels in the sera samples (n = 3). Data are mean±SD.*P < 0.05, ***P < 0.001.