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. Author manuscript; available in PMC: 2015 Sep 26.
Published in final edited form as: Nature. 2015 Jan 7;519(7544):477–481. doi: 10.1038/nature14107

Figure 1. SLC38A9 is a lysosomal component of the amino acid-sensing machinery controlling mTORC1.

Figure 1

a, Interactors of SLC38A9 identified by TAP–LC-MS/MS. Data shown are based on two independent experiments (n=2), each analysed in two technical replicates. b-g, Lysates from HEK293T cells transfected with the indicated tagged constructs or empty vector (−) (b, c, g), control (empty vector or shGFP) or shSLC38A9 transduced HEK293T (d) and HeLa (e), or K562 (f) cells were subjected to immunoprecipitation. PNGase-treated immunoprecipitates (IP) and protein extracts (XT) analysed by immunoblot with the indicated antibodies. Results are representative of two independent experiments (n=2) <: ST-HA-SLC38A9; *: non-specific band h-j, Confocal microscopy images of HeLa cells transfected with tagged SLC38A9 construct and immunostained with the anti-HA and LAMP1(h), EEA1(i) or Giantin (j) antibodies. Representative cells are shown. Scale bar, 10 μm.