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. Author manuscript; available in PMC: 2015 Sep 26.
Published in final edited form as: Nature. 2015 Jan 7;519(7544):477–481. doi: 10.1038/nature14107

Extended Data 6. The cytoplasmic N-terminal region of SLC38A9 binds the Ragulator/RAG GTPase complex through evolutionary conserved motifs.

Extended Data 6

a, Sequence alignment of the N-terminal cytoplasmic region (amino acids 1-112) of human, mouse, rat, Xenopus and zebrafish SLC38A9. Amino acids selected for deletion and motifs substituted to alanine are highlighted. Black and grey shading indicates > 60% amino acid sequence identity and similarity, respectively. b-c, HEK293T cells were transfected with the indicated tagged SLC38A9 constructs. Anti-HA immunoprecipitates and cell extracts were analysed by immunoblot. SLC38A9 mutant constructs are labelled with the number of the encoded amino acids (b) or with the amino acid motif substituted to alanine (c). Results are representative of two independent experiments (n=2).