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. Author manuscript; available in PMC: 2015 Sep 26.
Published in final edited form as: Nature. 2015 Jan 7;519(7544):477–481. doi: 10.1038/nature14107

Extended Data 10. Expression of SLC38A9 is required for amino acid-induced mTORC1 activation and is not affected by starvation.

Extended Data 10

a. HeLa were transfected with siRNA targeting SLC38A9 (SLC), LAMTOR1 (LT1) or non targeting control (CNTR). After 72h, cells were starved for 50 min in medium without amino acids and serum and then stimulated with amino acids in presence of insulin (1 μM). Cell lysates were analysed by immunoblot. Results are representative of three independent experiments (n=3). b-c, HEK293T cells were starved for the indicated times. SLC38A9 expression was analysed by quantitative PCR (b) and immunoblot (c). In (b) mean values ± s.d. from technical triplicates is shown. Results are representative of two independent experiments (n=2) <: SLC38A9; *: non-specific band.