Extended Data Figure 5. Analysis of RNA types, stem lengths, and sequence motifs at STAU1-target RNA duplexes.

a, Relative proportions of RNA types that were identified by hybrid reads, where each arm maps to the same (left) or different RNAs (right). b, Distribution of duplex stem lengths for 3′ UTR duplexes. c, Sequence motifs enriched at STAU1-target 3′ UTR duplexes when compared to surrounding regions of same 3′ UTRs (controls). d, Purine content is plotted within each arm of randomly selected 2291 3′ UTR duplexes that were detected using RNAfold²⁴ in mRNAs that don’t contain any STAU1-target hiCLIP duplex in their 3′ UTR, and surrounding sequence up to 40nt on each side. e, Purine content is plotted within each arm of 494 CDS-CDS STAU1-target duplexes and surrounding sequence up to 40nt on each side. f, Boxplots showing the frequencies of consecutive purine tracks in the 3′ UTR STAU1-target duplexes (boxplot on the left) and in the randomly selected 3′ UTR duplexes that were detected using RNAfold in mRNAs that don’t contain any STAU1-target hiCLIP duplex in their 3′ UTR (boxplot on the right).