Table 1. RT-PCR primers.
Gene | Primer sequence | Species | GenBank accession number | PCR product size (bp) |
---|---|---|---|---|
Enam | S 5’-TGGCAATGGACTTTACCCCTATC-3’ | Mouse | NM_017468.3 | 273 |
AS 5’-GCATCAGGCACAGTTGAGTTTGTAG-3’ | ||||
Amelx | S 5’-TGAGGTGCTTACCCCTTTGAAGTG-3’ | Mouse | NM_009666.3 | 216 |
AS 5’-GGAACTGGCATCATTGGTTGC-3’ | ||||
Ambn | S 5’-TTGAGCCTTGAGACAATGAGAC-3’ | Mouse | NM_009664.1 | 114 |
AS 5-’AAGTCCGTGCAACCATAAACTAT-3’ | ||||
Tuft1 | S 5’-CGGAACTGGTGTACCTTGGTG-3’ | Mouse | NM_011656.2 | 152 |
AS 5’-GGCATCATGGCATAGGTCTTC-3’ | ||||
Mmp20 | S 5’-TACGAAGTGGCTGAACGAG-3’ | Mouse | NM_013903.2 | 114 |
AS 5’-TGGGAACCCGAAGTCATA-3’ | ||||
Klk4 | S 5’-TTGCAAACGATCTCATGCTC-3’ | Mouse | NM_019928.1 | 228 |
AS 5’-TGAGGTGGTACACAGGGTCA-3’ | ||||
Odam | S 5’-GTCACATCCTCACCACAGCA-3’ | Mouse | NM_027128.2 | 160 |
AS 5’-GAGTTTCTGGAGCTGTGCCT-3’ | ||||
Amtn | S 5’-GGACCACTGAATGGACAGCA-3’ | Mouse | NM_027793.1 | 191 |
AS 5’-TCTGGTTTAGTGCCTGCCTG-3’ | ||||
DLX3 | S 5’-AGCCCAGTATCTGGCCTTG-3’ | Mouse | NM_010055.3 | 133 |
AS 5’-CGGCACCTCCCCATTCTTA-3’ | ||||
Gapdh | S 5’- CCAGCCTCGTCCCGTAGACA-3’ | Mouse | NM_008084.2 | 189 |
AS 5’- CCGTTGAATTTGCCGTGAGT-3’ |
As, antisense; S, sense.