. 2015 Mar 27;10(3):e0122115. doi: 10.1371/journal.pone.0122115

Table 2. Recognition of TcCA-2 epitopes by HLA-A*02:01 Chagas disease patients.

Peptide	Chagas disease patient ^a	INF-γ secretion range (pg/mL) ^b
TcCA-2_273-281	1/10	8.6
TcCA-2_442-451	2/10	5.8–6.9
TcCA-2_452-461	0/10	-
TcCA-2_489-498	0/8	-
TcCA-2_531-540	0/9	-
TcCA-2_550-559	0/9	-
TcCA-2_607-615	3/9	6.4–15.1
TcCA-2_657-666	2/9	6.1–6.7

K562-A2 cells were pulsed with 1 mM of each peptide for 1 h, and co-cultured with PBMCs from asymptomatic HLA-A*02:01 Chagas disease patients. Following 30 h of growth, IFN-γ secretion was measured in the supernatants. IFN-γ secretion level in K652 cells, not expressing the class I molecule, was subtracted from the values obtained in the assays with the K562-A2 cells loaded with peptides.

Responses were considered positive when the supernatant IFN-γ concentration of the peptide-stimulated cells was at least two folds the concentration of the un-stimulated cells (non-peptide).

^a Patients with positive response, in relation with the total number of patients per assay.

^b Range of IFN-γ secretion by patients with a positive response to TcCA-2 peptides.