Table 3. Cytokine-secretion and Granzyme B response to TcCA-2 epitopes by PBMCs from HLA-A*02:01 Chagas disease patients and healthy donors.
| Clinical | Code | IL6 | IFNγ | TNFα | GzB c | Clinical | Code | IL6 | IFNγ | TNFα | GzB c |
|---|---|---|---|---|---|---|---|---|---|---|---|
| form a | patient | (pg/mL) b | (SFC/106) | form a | patient | (pg/mL) b | (SFC/106) | ||||
| TcCA-2 273-281 | TcCA-2 607-615 | ||||||||||
| HD | 340 | 9 | 0 | 4 | 0 | HD | 340 | 6 | 0 | 0 | 0 |
| HD | 008 | 9 | -1 | 0 | -47 | HD | 008 | 22 | 3 | 0 | -70 |
| HD | 664 | 0 | -3 | 0 | 0 | HD | 664 | 0 | -3 | 0 | 0 |
| HD | 001 | 1 | 6 | -8 | 1 | HD | 001 | -6 | 1 | -14 | 0 |
| HD | 006 | 1 | 6 | 2 | -7 | HD | 006 | 69 | 13 | 5 | 7 |
| HD | 005 | -183 | -19 | -11 | -47 | HD | 005 | -77 | -21 | -2 | -47 |
| HD | 004 | -9 | 0 | 0 | 0 | HD | 004 | -5 | 0 | 0 | -7 |
| HD | 011 | -11 | 0 | -8 | -20 | HD | 011 | -12 | 0 | -8 | -7 |
| IND | 571 | 0 | 0 | 0 | 67 | IND | 535 | -5 | 0 | -1 | -7 |
| IND | 535 | -5 | 0 | -1 | -27 | IND | 265 | 1127 | 81 | 83 | -100 |
| IND | 388 | 36 | 2 | 7 | 33 | IND | 283 | 55 | 15 | 26 | -20 |
| IND | 365 | 43 | 1 | -6 | 7 | IND | 388 | 32 | 1 | -11 | 13 |
| IND | 510 | 1 | 0 | -1 | -20 | IND | 365 | 23 | 5 | 23 | 7 |
| IND | 917 | 18 | 7 | 11 | 13 | IND | 510 | -1 | 0 | 2 | 13 |
| CCC I | 861 | -4 | 2 | -2 | 8 | IND | 917 | -2 | 1 | -3 | -20 |
| CCC I | 884 | 0 | 0 | 0 | 7 | CCC I | 861 | -7 | 0 | -3 | -8 |
| CCC I | 456 | 0 | 0 | 0 | 13 | CCC I | 884 | 0 | 0 | 0 | 0 |
| CCC I | 770 | 22 | 0 | 4 | -7 | CCC I | 456 | 18 | 3 | 12 | 13 |
| CCC III | 509 | -4 | 0 | -5 | 0 | CCC I | 770 | 49 | 0 | 16 | 0 |
| TcCA-2 442-451 | CCC II | 142 | -4 | 0 | -15 | 53 | |||||
| HD | 340 | 11 | 2 | 3 | 1 | CCC III | 797 | 9 | -5 | 3 | 27 |
| HD | 008 | 31 | 1 | 15 | -73 | CCC III | 509 | -5 | 0 | -4 | 25 |
| HD | 664 | 0 | -3 | 0 | 1 | TcCA-2 657-666 | |||||
| HD | 001 | 18 | 4 | -6 | 0 | HD | 340 | 22 | 1 | 8 | 1 |
| HD | 006 | -2 | 2 | 2 | -7 | HD | 008 | 3 | -3 | -21 | -47 |
| HD | 005 | 16 | 5 | -1 | -40 | HD | 664 | 0 | -3 | 0 | 0 |
| HD | 004 | -15 | 0 | -1 | 0 | HD | 017 | 0 | 0 | 0 | 0 |
| HD | 011 | 54 | 8 | 10 | -20 | HD | 001 | 1 | -2 | -6 | 0 |
| IND | 535 | -6 | 0 | -1 | -13 | HD | 006 | -5 | 3 | 1 | 0 |
| IND | 265 | 1641 | 204 | 160 | -13 | HD | 005 | -58 | 0 | -4 | -40 |
| IND | 283 | 80 | 12 | 35 | 47 | HD | 004 | -12 | 4 | -1 | -7 |
| IND | 388 | 21 | 1 | -10 | 0 | HD | 011 | -4 | 0 | 0 | -7 |
| IND | 365 | 7 | 0 | 15 | 27 | IND | 571 | 0 | 1 | 0 | 83 |
| IND | 510 | 1 | 0 | 1 | 0 | IND | 535 | -5 | 0 | -2 | 67 |
| IND | 917 | -13 | -1 | -4 | 40 | IND | 388 | 2 | 2 | -5 | 7 |
| CCC I | 861 | -4 | 0 | -3 | 0 | IND | 365 | 10 | 2 | 14 | 0 |
| CCC I | 884 | 0 | 0 | 0 | 7 | IND | 510 | -3 | 0 | 1 | -7 |
| CCC I | 456 | 46 | 4 | 6 | -7 | IND | 917 | -17 | -1 | -17 | -10 |
| CCC I | 770 | 3 | -2 | -11 | 13 | CCC I | 861 | -4 | 0 | -2 | 50 |
| CCC II | 142 | -3 | 2 | -20 | 80 | CCC I | 884 | 0 | 0 | 0 | 0 |
| CCC III | 797 | 31 | 3 | 7 | 7 | CCC I | 770 | 1 | 0 | -6 | -7 |
| CCC III | 509 | -4 | 0 | -5 | 50 | CCC III | 509 | -4 | 0 | -5 | 0 |
a HLA-A*02:01 Chagas disease patients in asymptomatic phase (IND) and cardiac forms (CCC). HD: HLA-A*02:01 healthy donors. The cytokine secretion and the number of GzB-secreting cells were calculated by subtracting background levels or the number of spots in control wells (non-peptide) from the levels measured or the number of spots obtained in the stimulated cultures. Responses were considered positive (in bold) when:
b The cytokine concentration is at least three folds the non-peptide control value and ≥15 pg/mL for IL-6 and ≥ 5 pg/mL for IFN-γ and TNF-α.
c The number of SFC per million was ≥20 and at least twice the value found in the background control wells.