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. 2015 Mar 27;10(3):e0121447. doi: 10.1371/journal.pone.0121447

Fig 1. Detection of BmCPV polyhedrin by RT-PCR in persistently and pathogenically infected silkworm tissues and eggs.

Fig 1

Polyhedrin RT-PCR was performed using samples derived from larvae persistently and pathogenically infected (a) at the 2nd or (b) the 4th instar (2c, 2inf, 4c and 4inf samples), as well as from Daizo and P50 silkworm strain-derived eggs. Polyhedrin PCR product with a size of 689 nucleotides was obtained after 40 cycles of PCR. Abbreviations: BW: body wall, MG: midgut, MGC: midgut content, F: feces, (+): positive control (BmCPV polyhedra), (-): negative control (water sample).