FIG. 3.

Proliferation of human HCT116 colon cancer cells in hydrogel particles. (a) Time-series image of the growth of a single cell in a hydrogel particle. Note that at t = 3.5 h, daughter cells begin to shrink back into their maternal cell (scale bar = 10 μm). (b) Percentage of living cells at different time when cells are cultured in petri dish and hydrogel particles. N₀ is the number of living cells at t = 0 h, and N is number of living cells at t = n hours (n = 24, 48, 72, 96, and 120). (c) The net growth rate (n_n − n₀)/n₀ × 100%) of cells encapsulated in hydrogel particles. n_n is the number of particles containing 2 or 3 cells quantified at day n, n = 1, 2, 3, and 4. n₀ is the number of particles containing 2 or 3 cells collected at day 0. Error bar is presented as standard deviations of the mean. (Multimedia view) [URL: http://dx.doi.org/10.1063/1.4916508.1] Download video file ^{(461.2KB, mp4)} DOI: 10.1063/1.4916508.1