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. 2015 Mar 27;10(3):e0108874. doi: 10.1371/journal.pone.0108874

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© 2015 Han et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) Detection of glycogen in the cytoplasm of MSCs subjected to the liver differentiation protocol was demonstrated by PAS staining. PAS-positive substances stain pink in the cytoplasm of cells. (B) The number of PAS-positive cells is expressed as percentage of the total number of counted cells and was significantly higher in Oct4/Sox2-ATMSCs than that of RFP-ATMSCs. The experiments were repeated at least three times and similar findings were observed. Data represent the mean ± SD of three independent experiments. Statistical analysis was performed by student t-test (significant, *p < 0.05).