Table 1. Primer sequences for RT-PCR amplification of target genes.
Gene | Primer | Gene Bank Accession number | Product Size (bp) | |
---|---|---|---|---|
Oct4 | F | 5’-TGCAGAAAGAACTCGAGCAA-3’ | NM_002701.4 | 1082 |
R | 5’-ACACTCGGACCACATCCTTC-3’ | |||
Sox2 | F | 5’-AGAACCCCAAGATGCACAAC-3’ | NM_003106.3 | 466 |
R | 5’-ATGTAGGTCTGCGAGCTGGT-3’ | |||
AFP | F | 5’-GGCAGCCACAGCAGCCACTT-3’ | NM_001134.2 | 276 |
R | 5’-TGCAGCGCTACACCCTGAGC-3’ | |||
ALB | F | 5’-CAACTATGTCCGTGAGCTTGGA-3’ | NM_000477.5 | 339 |
R | 5’-GTGGTCGGTGCTGGTCTATATG-3’ | |||
transferrin | F | 5’-AGGGCCATTGCGGCAAACGA-3’ | DQ923758.1 | 240 |
R | 5’-GTTCCACCCAGCGGAGGTGC-3’ | |||
GAPDH | F | 5’-AAGTGGATATTGTTGCCATC-3’ | NM_002046.4 | 454 |
R | 5’-ACTGTGGTCATGAGTCCTTC-3’ |