Table 1. Primers used for amplification and sequencing of the entire penton base, hexon, and fiber gene.
Primer | Sequences (5′-3′ orientation) | Position a |
---|---|---|
HAdV-2-Penton-1F | GTGTGGGAGGACGATGACTC | 13,961–13,980 |
HAdV-2-Penton-1R | GTTGGTCGGAGCGCTTCTT | 15,948–15,966 |
HAdV3-Penton-1F | GGGCGCATGTTGTAAAAGTAA | 13,803–13,823 |
HAdV3-Penton-1R | GGTGCTGGGTAGAGCGTATG | 15,630–15,649 |
HAdV4-Penton-1F | ATGTGGGACGATGAGGATTC | 13,567–13,586 |
HAdV4-Penton-1R | GTCAATGACGGCGTCCAC | 15,608–15,625 |
HAdV7-Penton-1F | GATGATAGCAGCGTGTTGGA | 13,699–13,718 |
HAdV7-Penton-1R | GGTGCTGGGTAGAGCGTATG | 15,595–15,614 |
HAdV55-Penton-1F | GATGATAGCAGCGTGTTGGA | 13,515–13,534 |
HAdV55-Penton-1R | CACGGGATGTTGGGTAGAAC | 15,442–15,461 |
HAdV-2-Hexon -1F | AAGCACACTGAACAGCATCG | 18,690–18,709 |
HAdV-2-Hexon -1R | CACCACTCGCTTGTTCATGT | 20,393–20,412 |
HAdV-2-Hexon -2F | AGATGAAACTTTTGCAACACGT | 20,211–20,232 |
HAdV-2-Hexon -2R | CGTATTGACTATGGCGCAGG | 21,893–21,912 |
HAdV3-Hexon-1F | AGTACTCTGAACAGCATCGT | 18,258–18,277 |
HAdV3-Hexon -1R | TAGGTGGCGTGTACTTGTAA | 19,860–19,879 |
HAdV3-Hexon-2F | ACCGATGACGCTAATGGATG | 19,695–19,714 |
HAdV3-Hexon -2R | TATGGCGCAGGCGAGCTTGT | 21,415–21,434 |
HAdV4-Hexon -1F | TGACGCACACGGACGAACC | 17,773–17,791 |
HAdV4-Hexon -1R | AGAGGGCAACATTGGCATAG | 19,545–19,564 |
HAdV4-Hexon -2F | ATGGTGTGGAGGATGAATTG | 19,334–19,353 |
HAdV4-Hexon -2R | ACCGGCCGTATTGACGAT | 21,138–21,155 |
HAdV7-Hexon -1F | CTGAACAGCATCGTGGGTCT | 18,219–18,238 |
HAdV7-Hexon -1R | ACTCGCCCGTTCATGTACTC | 19,837–19,856 |
HAdV7-Hexon -2F | CGTCGAGGATGAACTGCCTA | 19,560–19,579 |
HAdV7-Hexon -2R | CAGTGTTGACTATGGCGCAG | 21,363–21,382 |
HAdV55-Hexon -1F | GTGCAAAGTGTAAAACGCCG | 18,085–18,104 |
HAdV55-Hexon -1R | GGTGGTGGTTGAATGGGTTG | 19,810–19,829 |
HAdV55-Hexon -2F | ATACACCCCGTCCAATGTCA | 19,672–19,691 |
HAdV55-Hexon -2R | CGCTTATCGTAGGTTCCCAA | 21,194–21,213 |
HAdV-2-Fiber-1F | CCTTTCCTTCCTCCCAACTC | 30,902–30,921 |
HAdV-2-Fiber-1R | TGTGGTGGTGGGGCTATACT | 32,861–32,880 |
HAdV-3-Fiber-1F | CTTCCTACCAGCAGCACCTC | 31,228–31,247 |
HAdV-3-Fiber-1R | CGTGGGGAGAGATTGGTGTA | 32,419–32,438 |
HAdV-4-Fiber-1F | CTTCCCAGCTCTGGTACTGC | 31,349–31,368 |
HAdV-4-Fiber-1R | GGAGGGTGGAGGGAAAATAA | 32,849–32,868 |
HAdV-7-Fiber-1F | GAAATTTTCTCCCAGCAGCA | 31,100–31,119 |
HAdV-7-Fiber-1R | ATTGGCTCGCTTTGAAACTG | 32,392–32,411 |
HAdV-55-Fiber-1F | GAAATTTTCTCCCAGCAGCA | 30,626–30,645 |
HAdV-55-Fiber-1R | AGATTGGCTCGCTCTGAAAC | 31,921–31,940 |